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HTS-Microplates

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Standard Microplate Footprint

The manufacture of user-friendly products is one of our most important goals. All microplates manufactured by Greiner Bio-One have a uniform footprint which is conform to the recommendation of the American National Standards Institute (ANSI) (ANSI/SBS 1-2004).

 

Material

Polypropylene (PP) and polystyrene (PS) are the standard materials used to manufacture the majority of microplates. Polystyrene is a highly clear polymer with excellent optical properties which makes it ideal for precise optical measurements. Polystyrene is also characterised by its ability to bind biomolecules, such as proteins, and it is therefore often used for manufacturing immunological products. Polystyrene is suitable for work with cell cultures.

Polypropylene is characterised by its excellent chemical and thermal stability. It is the ideal polymer for storage vessels or microplates. Polar molecules, such as proteins or DNA, are binding less to polypropylene than to polystyrene.

In addition to polystyrene and polypropylene microplates, Greiner Bio-One manufactures microplates made from different polyolefins. These polyolefins are characterised by their low level of autofluorescence, exceptionally high clarity, especially in the UV range, and greater chemical stability when compared with polystyrene.

 

uClear and UV-Star

The move from isotopic to non-isotopic assays (fluorescence / luminescence),  and new applications in genomics and microscopy, has created a demand for clear bottom plates, microplates with pigmented walls and thin film bottoms. The development of a completely new and patented processing technique has made it possible for us to produce microplates with ultra-thin films, without the use of adhesives or solvents  the µClear and UV-Star products.

Polarised light is either not depolarised (UV-Star) or is only depolarised to a slight degree (µClear) and the autofluorescence of the microplates is minimised.

 

MICROLON, FLUOTRACTM, LUMITRACTM

MICROLON, FLUOTRACTM, and LUMITRACTM stand for the quality of our immunology products. MICROLON are clear microplates for transmission measurements. FLUOTRACTM are black microplates for fluorescence measurements. LUMITRACTM are white microplates for luminescence measurements. MICROLON 600, FLUOTRACTM 600 and LUMITRACTM 600 are high binding polystyrene surfaces that have been specifically treated to provide an increased protein binding.

MICROLON 200, FLUOTRACTM 200 and LUMITRACTM 200 are medium binding (med. binding) polystyrene surfaces. The polystyrene surface of a medium binding microplate is more hydrophobic than the surface of a high binding microplate, and therefore tends to be more suitable for non-polar proteins and peptides.

 

Non-binding Surfaces

Non-binding surfaces from Greiner Bio-One are characterised by their low binding capacity for biomolecules such as DNA, RNA, peptides and proteins. The repellent property of the non-binding surfaces for biomolecules can be advantageous in biochemical assays by increasing the sensitivity, reducing the background and improving the signal-to-noise ratio.

Achieved through a chemical modification of the resin rather than a resin mixture with potential to leach, the non-binding surface from Greiner Bio-One is stable under common assay conditions and does not degrade during short-term storage.

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