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High Performance Liquid Chromatography

HPLC SYSTEM S 500 SERIES

The Sykam ROUTINE HPLC System S 500 is intended for all routine analysis. The system configuration is highly variable and several upgrade options make this system suitable for the whole range of analytical applications.

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SYKAM S 1125 HPLC Pump

The Sykam S 1125 HPLC Pump System is a very flexible and powerful HPLC solvent delivery system. Its modular setup makes the S 1125 one of the most versatile pumps systems on the market. The possible configurations include an Isocratic or Quanternary Gradient Pump.

Stepper Motor

The S 1125 is driven by a high-power stepper motor. The stepper motor has a much better resolution in the low-flow range than a conventional DC motor. 

Active Mixer

The S 1125 low pressure gradient module has an active mixer to achieve highly precise and accurate gradient results.

Lubrication

The S 1125 camshaft is constantly lubricated within a sealed chamber to guarantee long lifetime and low maintenance.

Dual-Piston Pumphead

The S 1125 pumps use a dual-piston pumphead for low pulsation. Together with electronic pressure compensation the S 1125 pumps are suitable for all analytical tasks in HPLC and GPC

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S 5250 AUTOSAMPLER

The Sykam S 5250 Sample Injector System is a very flexible and powerful HPLC autosampler with excellent reproducibility and linearity properties. Variable vial racks and adaptors for microtiter plates as well as a multitude of firmware options make this system highly adaptable and suitable for any analytical application.

 

Robust Design

Durable X/Y/Z-Sampling

The S 5250 Sample Injector System features a mechanically durable X/Y/Z-Sampling-Mechanic designed for long life operation. The self-lubricating bearings keep the routine maintenance at a minimum and avoid troubles caused by dusty environments. High precision stepper motors drive the X/Y axis for accurate positioning. Microstepping mode enables a high resolution for the syringe dosing and vial positioning.

Dual-Needle Design

The Dual-Needle design of the S 5250 Sample Injector System avoids system blockages caused by septum particles injected into the system. The ventilation needle pierces the septum before the injection needle moves into the sample vial (see figure on the right). As the more fragile injection needle does not need to pierce the vial septum, stronger vial caps or plastic vials can be used without problems.

Performance

The Sykam S 5250 Sample Injector System offers multiple injection modes depending on application and sample needs. Besides fixed loop overfilling and variable volume injection the instrument offers a Zero- Waste injection mode for injecting very small sample amounts by moving the sample into the middle of the sample loop. 

Linearity and injection precision can be optimized for any volume by different sample loops and syringe sizes.

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Detectors

Sykam offers the full range of HPLC detectors from routine UV/Vis Detectors and RI Detectors to sophisticvated Electrochemical, Conductivity and Fluorescence Detectors. With this range Sykam can offer a solution for any application.

 

 

 

S 3245 UV/Vis DETECTOR

The Sykam S 3245 UV/Vis Detector is a variable wavelength UV/Vis detector for routine analysis and sophisticated research. The dual lamp design offers a wavelength range of 190 – 800 nm with a low baseline noise. The front-accessible flowcell can be easily  exchanged, as well as the lamps which are accessible through a side panel in the  instruments housing.

Integrated Wavelength Program

The S 3245 UV/Vis Detector features a wavelength program to change the selected wavelength over time. With this feature the optimum wavelength can be selected for each analyzed substance according to its retention time.

Integrated Peak Detector

The integrated Peak Detector works as a basic fraction collector. The peak detection level can be freely programmed for peak start and peak end to enhance the collection purity. An integrated 24V output for switching a solenoid valve is used for the fraction collection, which is automatically operated with a selectable time delay.

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S 3345 PDA DETECTOR

The Sykam S 3345 UV/Vis Detector is a photodiode-array (PDA) detector for routine analysis and sophisticated research. The dual lamp design offers a wavelength range of 190 - 720 nm (256 Diodes) or 190 - 1015nm (1024 diodes) with a low baseline noise. The front-accessible flowcell can be easily exchanged as well as the lamps which are accessible through a side panel in the instruments housing.

4-Channel UV Detector

The S 3345 PDA Detector features 4-Wavelength channels to measure chromatograms at 4 different wavelengths at the same time. With this feature the optimum wavelength can be selected for each analyzed substance.

Integrated Peak Detector

The integrated Peak Detector works as a basic fraction collector. The peak detection level can be freely programmed for peak start and peak end to enhance the collection purity. An integrated 24V output for switching a solenoid valve is used for the fraction collection, which is automatically operated with a selectable time delay.

 

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S 3585 REFRACTIVE INDEX DETECTOR

The Sykam S 3585 Refractive Index Detector offers the sensitivity, stability and reproducebility required for optimal RI detection.

 The thermal isolated optic with a countercurrent heat exchanger and with its programmable temperature control, results in an extremly stable baseline and an optimal Signal/Noise ratio.

The Sykam S 3585 Refractive Index Detector provides autopurge and autozero capabilities, as well as RS232 communication to acquire data directly without using any external interface.

Sykam S 3585 Refractive Index Detectors are available for :-

  • micro
  • analytical
  • semipreparative mode
 

S 4120 COLUMN OVEN

The Sykam S 4120 Column Oven is a contact heat transfer oven for high temperature stability and accuracy. The columns are mounted inside the column oven in optimized coumn holder which enclosed the complete column to get best temperature transfer between the heater and the column. Up to three 350mm columns can be mounted at the same time.

Heating

The Sykam S 4120 Column Oven standard version features a high temperature controller for stable column temperatures of +30oC up to +150oC. The temperature accuracy is within 0.1oC.

Heating/Cooling

 The Sykam S 4120 Column Oven is also available with active Heating/Cooling with Peltier technique. The temperature range is +5oC up to +100oC. The Heating/Cooling unit uses the same efficient controller as the basic version with temperature accuracy better than 0.1oC

Temperature Time Program

The Heating/Cooling variant offers an optional Temperature Time Programm for stand-alone operation.

Integrated Valve

The Sykam S 4120 Column Oven offers the option to include an automatic switching valve of the Sykam S 6000 Valve Series, for example the S 6070 Column Selection Valve, but all S 6000 Valves can be integrated.

Leakage Sensor

The S 4120 Column Oven offers a high sensitive Leakage Sensor which detects the vapors of organic solvents.

Temperature Fuse

Besides a Leakage Sensor the S 4120 offers a temperature fuse which shuts down the unit when the temperature becomes too high, because of an electronic detect.


S 7515 VACUUM DEGASSER

The Sykam S 7515 Vacuum Degasser is an online degasser system with high efficiency. Dissolved gases are removed from the solvents by applying vacuum to a semi-permeable membrane.

High Efficiency

The high efficient Teflon-AF. capillary has a much higher efficiency than a normal Teflon capillary of similar size. This allows the usage of a smaller length of capillary to reduce the dead volume of the system considerably.

 

2 Operation Modes 

The S 7515 can be run either with constant speed or in Hysteresis Mode, which switches the vacuum pump on or off.

5-Year Membrane Warranty

The S 7515 vacuum pump uses a membrane for creating the vacuum. This membrane is made of a specific Teflon material specifically designed for fast movements. Sykam offers a 5-year warranty on the lifetime of this membrane.

Multi-Channel

The S 7515 Vacuum Degasser is available as 1-Channel, 2-Channel, 3-Channel, or 4-Channel version. Each solvent channel can be used for a different solvent. Several channels can be used in series to increase the efficiency even more.